Journal: Cancer Cell International

Article Title: HOPX is a tumor-suppressive biomarker that corresponds to T cell infiltration in skin cutaneous melanoma

doi: 10.1186/s12935-023-02962-2

Figure Lengend Snippet: HOPX inhibits proliferation, migration, and invasion and induces apoptosis and cycle arrest in SKCM cell lines. ( A ) The overexpression of HOPX in A375 cells was confirmed by RT‒qPCR and Western blotting ( B ). ( C ) A CCK-8 assay was used to detect the proliferation of A375 cells. ( D ) A wound healing assay was used to detect the migration of A375 cells. ( E - H ) Transwell assays were used to detect the migration/invasion of A375 cells. ( I - J ) The apoptosis and cell cycle ( K - L ) of A375 cells were detected by flow cytometry

Article Snippet: In addition, the FastKing One Step RT‒qPCR Kit (SYBR) (TIANGEN, China) and an Eppendorf Quantitative PCR Instrument (Eppendorf, China) were used for all RT-qPCRs.

Techniques: Migration, Over Expression, Western Blot, CCK-8 Assay, Wound Healing Assay, Flow Cytometry

Journal: Nature

Article Title: Progenitor identification and SARS-CoV-2 infection in human distal lung organoids

doi: 10.1038/s41586-020-3014-1

Figure Lengend Snippet: a, b, Distal lung organoid modelling of H1N1 influenza infection. a, Merged transmission and GFP confocal images of purified basal (left) and purified AT2 organoids (right) 12 h after infection with PR8-GFP H1N1 influenza virus, quantified by FACS for % GFP+ cells. Bar plot represents the mean percentage of infected cells from three technical replicates, P = 0.57, Chi-square test. Scale bars = 50 μm. b, Viral genome quantitation over time of mixed distal lung organoid culture supernatants subjected to initial infection of wild-type H1N1 at an estimated multiplicity of infection (MOI) of 0.01, qRT–PCR, data represent the mean of three independent experiments ± s.e.m. c, d, Lectin staining with M. amurensis (a2–3) and S. nigra (a2–6) lectins or no lectin negative controls to characterize sialic acid residues which serve as surface molecules for influenza virus host cell entry. AT2 organoids (c) and basal organoids (d). Scale bar = 25 μm. e, Dose response curves for two different classes of antiviral drugs on influenza infectivity and replication. The nucleoside analogue FdC demonstrated dose dependent activity with IC50 of 340 nM as compared to neuraminidase inhibitor zanamivir, which only impairs viral shedding but not infectivity and replication. n = 3 per condition, data represents mean ± s.e.m. f, Fluorescence micrograph of multiwell screening of selected various antiviral agents after H1N1 PR8-GFP organoid infection in 48 well format. FdC = nucleoside analogue 2′-deoxy-2′-fluorocytidine. Cpd = compound #.

Article Snippet: The Brilliant II SYBR Green QRT–PCR 1-Step Master Mix (VWR) was used to convert RNA into cDNA and to amplify specific RNA regions on the CFX96 Touch real-time PCR detection system (Bio-Rad).

Techniques: Infection, Transmission Assay, Purification, Quantitation Assay, Quantitative RT-PCR, Staining, Activity Assay, Fluorescence